Peroxidase-catalysed interfacial adhesion of aquatic caddisworm silk

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Peroxidase-catalysed interfacial adhesion of aquatic caddisworm silk.

Casemaker caddisfly (Hesperophylax occidentalis) larvae use adhesive silk fibres to construct protective shelters under water. The silk comprises a distinct peripheral coating on a viscoelastic fibre core. Caddisworm silk peroxinectin (csPxt), a haem-peroxidase, was shown to be glycosylated by lectin affinity chromatography and tandem mass spectrometry. Using high-resolution H2O2 and peroxidase...

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Connecting caddisworm silk structure and mechanical properties: combined infrared spectroscopy and mechanical analysis

The underwater silk of an aquatic casemaking caddisfly larvae (Hesperophylax occidentalis) is viscoelastic, and displays distinct yield behaviour, large strain cycle hysteresis and near complete recovery of its initial strength and stiffness when unloaded. Yield followed by a stress plateau has been attributed to sequential rupture of serial Ca(2+)-cross-linked phosphoserine (pS) β-domains. Spo...

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Interfacial propulsion by directional adhesion

The rough integument of water-walking arthropods is well known to be responsible for their water-repellency [1, 2, 3, 4]; however, water-repellent surfaces generally experience reduced traction at an air-water interface [5, 6, 7, 8]. A conundrum then arises as to how such creatures generate significant propulsive forces while retaining their water-repellency. We here demonstrate through a serie...

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Freestanding protein films are interesting for many applications ranging from microencapsulation to tissue engineering. Here, the authors use interfacial rheology to characterize the adsorption kinetics and the rheology of spider-silk films formed at an oil water interface. The high surface activity of the engineered spider-silk proteins results in a fast formation of highly stable films, which...

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ژورنال

عنوان ژورنال: Journal of The Royal Society Interface

سال: 2015

ISSN: 1742-5689,1742-5662

DOI: 10.1098/rsif.2015.0710